adeno-associated viruses (aavs, encoding chr2, or gfp or cre) or rabies virus (encoding gfp)  (Addgene inc)

Journal: Nature neuroscience

Article Title: Laminar-specific Scaling Down of Balanced Excitation and Inhibition in Auditory Cortex by Active Behavioral States

doi: 10.1038/nn.3701

Figure Lengend Snippet: Changes of activity of PV neurons between different behavioral states. ( a ) Confocal image of a brain section showing ChR2-EYFP expression in the A1 region. ( b ) Left, sample loose-patch recording trace showing a train of evoked spikes of a PV cell to blue LED illumination (marked by the blue bar). Right, sample spike response of the same cell to CF-tone stimulation. Top inset, schematic drawing of loose-patch of a cell. Bottom inset, superimposed 500 individual spikes (black) and the average spike shape (red) of the cell. ( c ) Spike waveforms of four more PV and excitatory cells. ( d ) Plot of peak to trough amplitude ratio versus trough-to-peak interval for average spike waveforms of PV and excitatory cells recorded with loose-patch methods. Optogenetically identified PV cells were labeled by open circles. ( e ) Summary of spontaneous firing rates of recorded L2/3 PV cells in different states. Bar = s.d.. N = 8 cells. ** P = 0.0044, t = 4.140, paired t -test. ( f ) CF-tone evoked spike numbers for the cells in ( e ). ** P = 0.0047, t = 4.086, paired t -test. ( g ) Summary of spontaneous firing rates for PV cells recorded in L4. P = 0.5013, t = −0.7243, paired t -test, n = 6 cells. ( h ) CF-tone evoked spike numbers for the cells in ( g ). P = 0.7286, t = 0.3671, paired t -test.

Article Snippet: Adeno-associated viruses (AAVs) encoding ChR2 were purchased from the University of Pennsylvania Viral Vector Core: AAV2/9.EF1α.DIO.hChR2(H134R)-EYFP.WPRE.hGH (Addgene 20298).

Techniques: Activity Assay, Expressing, Labeling